Biochrom Bio 30+ General Lab Use Amino Acid Analyzer Systems

aaa 30+ amino acid analyzer biochrom

Biochrom Bio 30+ General Lab Use Amino Acid Analyzer Systems

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Biochrom’s Bio 30+ Series is the gold standard in amino acid analysis trusted by diagnostic laboratories. It employs a classical method based on ion exchange chromatography with post-column derivatization using Ninhydrin. This system enables qualitative and quantitative analysis of 56 amino acids and derivatives, including argininosuccinic acid, alloisoleucine, and sulfocysteine. The Biochrom Bio 30+ Physiological System uses a combination of 5 lithium citrate buffers to achieve a full amino acid profile in under 2 hours using the new accelerated method.

 

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biochrom
Bioprocessing
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Why you need a dedicated instrument for amino acid analysis: 

Conventional HPLC systems face challenges in amino acid analysis due to sample complexity and unique chemical properties. A dedicated AAA system provides a reliable, consistent, and reproducible solution without the need for method development. Its pre-set and extensively tested configuration allows 24/7 routine use with minimal user intervention. Efficient screening of numerous samples for specific amino acids can be swiftly conducted around the clock using concise methods.

 

System suitable for: 

  • Amino acid analysis for research and general lab use on various physiological sample matrices
  • Quality control and compositional analysis in biosciences and pharmaceutical industries

 

Specific applications: 

  • Detection of up to 56 amino acids in physiological samples
  • Protein and peptide analysis for pharmaceutical applications
  • Food, beverages, plant extracts, fermentation products, culture media, nutraceuticals and infusion fluids

 

Biochrom 30+ Series of Amino Acid Analyzers are customizable.

Please contact your sales representative or distributor for system ordering information

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A Dedicated Amino Acid Analysis System 

Accurate: Superb separation, accurately identifies and quantifies free amino acids, as well as the amino acid composition of proteins and peptides.
Reliable: Minimal matrix interference and near baseline separation. Automated derivatization of amino acids maximizes reproducibility.
Fast: Rapid protocols that increase sample throughput by cutting analysis time.
Economical: The column cleaning and repacking service, gives columns an almost indefinite life and cuts the cost of ownership.
Fully Automated: Total PC control allows 24/7 operations.
Versatile: Wide variety of sample types compatible: Protein hydrolysates from various food and feedstuff samples or synthetic drugs, nutraceuticals, beverages.
Easy to implement: No lengthy method development is required as this well characterized system is established worldwide. All customers have their instrument optimized to their application directly from the factory.
Convenient: Interchangeable sample trays allow samples to be prepared in advance and stored refrigerated.
Robust: Advanced fluidics and robust hardware mean easy maintenance for users, backed by the reassurance of Biochrom's dedicated service and support team.
Flexible: Integrated software, choice of different methods to adapt to the user's needs. Automatic sample set-up. Networking and exporting of data.
Compact: Bench top design requiring minimum lab space.

 

Screening Methods

Choose from 3 full screening methods according to your business needs:

Choice of Screening Methods
System Use Minutes (to Arginine)
Biochrom Bio 30+ Physiological Accelerated LC System  When speed is a decisive factor, with little compromise on the peak resolution 120 min
Biochrom Bio 30+ Physiological High Performance LC System The best compromise between speed, resolution and column life 170 min
Biochrom Bio 30+ Physiological High Resolution LC System When 100% baseline separation and a maximum number of amino acids separated are required. Ideal for research and low sample throughputs 210 min

 

Instrument Typical Package 

The instrument package consists of the Analyzer (electronics unit, pumps, and detection system) plus:

  • Cooled Autosampler
  • Column kit: analytical column and top-up ion exchange resin, prewash column and top-up ion exchange resin
  • Ninhydrin
  • Buffer Chemical Kit suitable for up to 170 analyses (high performance method)
  • Spare parts and consumables kit
  • Calibration Standard
  • Hardware control and data handling software
  • Desktop PC, Windows 10 64-bit Professional, LED widescreen monitor
  • User Manual (PDF format)
  • Power cables

For buffer and chemicals information, consult the Consumables tab below.

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Instrument Specifications
Specification Data
Reproducibility Area: <0.5% RSD at 10 nanomoles
Retention time: <0.1% RSD
Pumps Two dual piston pumps with integrated pressure transducer and drain valve
Ceramic pump head, sapphire pistons and valves
Electronically controlled piston wash
Operating Pressures Buffer pressure: maximum 150 bar
Ninhydrin pressure: maximum 24 bar
Analytical Column High pressure PEEK column packed with Ultropac 8 cation exchange resin Peltier heating/cooling system
Sample Injection 3 injection modes (full loop, partial loop and micro), 84 position autosampler
Sample volumes from 1 µL to 5000 µL (200 µL loop supplied as standard)
Temperatures Column temperature variable between 20°C and 99°C
Reaction Coil temperature adjustable between 40°C and 145°C (135°C is optimum)
Software Phoenix Integrated Control Software
Data Apex Clarity or Agilent OpenLAB CDS EZChrom Edition
Photometer Single flow cell with optical beam splitter with detection at 440 nm and 570 nm
Weight and Dimensions Main System: 50 kg (110 lb.) 48 x 59 x 57 cm (19 x 23 x 22 in.)
Autosampler: 21kg (46 lb.) 30 x 57.5 x 36 cm (12 x 23 x 14 in.)
Required Services Oxygen free Nitrogen gas (99.99%) or Argon gas regulated to 5 bar
Drainage facility
240V/100V, 50Hz/60Hz, 300VA mains supply
Safety Systems

Automatic shut down and reaction coil flushing in the event of:

  • Power failure
  • Photometer lamp failure
  • Low ninhydrin, low buffer, low coil or low nitrogen pressures
  • High coil or column temperatures, high ninhydrin, high buffer or high coil pressures

 

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Related Documents
Biochrom 30+ Amino Acid Analyzer System brochure.pdf DOWNLOAD BROCHURE
Material Safety Data Sheet (MSDS) View MSDS
Certificates of Analysis View Certificates of Analysis

 

Product name
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EZ Nin Reagent Kit 2L NC
Item #:
80-6003-12

Sufficient for up to 90 runs (Protein Hydrolysate System) or 45 runs (Physiological Fluid High Performance System)
Includes: EZ Nin Reagent ready-to-use 2L bottle, In-line filter, Quick Start Guide

EZ Nin Reagent Kit 4L NC (2 x 2L)
Item #:
80-6003-13

Sufficient for up to 180 runs (Protein Hydrolysate System) or 90 runs (Physiological Fluid High Performance System)
Includes: EZ Nin Reagent ready-to-use 2x 2L bottle, 2x In-line filter, Quick Start Guide

EZ Nin Reagent Kit 8L NC (4 x 2L)
Item #:
80-6003-14

Sufficient for up to 360 runs (Protein Hydrolysate System) or 180 runs (Physiological Fluid High Performance System)
Includes: EZ Nin Reagent ready-to-use 4x 2L bottle, 4x In-line filter, Quick Start Guide

Ultra Ninhydrin Kit 2L NC
Item #:
80-6003-10

Sufficient for up to 90 runs (Protein Hydrolysate System) or 45 runs (Physiological Fluid High Performance System)
Includes Ultra Ninhydrin Solution 2L, Ultrosolve Plus Solution, In-line Filter

Ultra Ninhydrin Kit 8L NC (4 x 2L)
Item #:
80-6003-11

Sufficient for up to 360 runs (Protein Hydrolysate System) or 180 runs (Physiological Fluid High Performance System)
Includes 4x Ultra Ninhydrin Solution 2L, 4x Ultrosolve Plus Solution, 4x In-line Filter

Lithium Buffer 1 NC
Item #:
80-6003-01

2 L, pH 2.80

Lithium Buffer 2 NC
Item #:
80-6003-02

2 L, pH 3.00

Lithium Buffer 3 NC
Item #:
80-6003-03

2 L, pH 3.15

Lithium Buffer 4 NC
Item #:
80-6003-04

2 L, pH 3.50

Lithium Buffer 5 NC
Item #:
80-6003-05

2 L, pH 3.55

Lithium Buffer 6 NC
Item #:
80-6003-06

1 L

Lithium Loading Buffer NC
Item #:
80-6003-07

2 L, Ph 2.20

Physiological Fluid amino acid standard, 2.5mM
Item #:
80-6002-80

This ISO 17034 Reference Material (RM) is manufactured and verified in accordance with systemISO17034 and ISO17025 requirements. The analyte concentrations are verified by a quality control laboratory and the true value and uncertainty values at the 95% confidence level are reported for each analyte.

This is supplied in a screw cap glass vial containing 5 mL of standard solution in a 0.1 N Hydrochloric Acid matrix. A Certificate of Analysis is provided with each vial. Store Refrigerated (2o to 8oC) Do Not Freeze

Documents:
Lithium Buffer Start-up Kit (no ninhydrin reagent included)
Item #:
80-6003-17

Sufficient for up to 170 runs (High Performance System) Includes 2x Buffer 1, 1x Lithium Buffer 2, 1x Lithium Buffer 3, 1x Lithium Buffer 4, 2x Lithium Buffer 5, 1x Lithium Regeneration Buffer 6

Lithium Buffer Routine Kit (no ninhydrin reagent included)
Item #:
80-6003-18

Sufficient for up to 170 runs (High Performance System) Includes 3x Buffer 1, 1x Lithium Buffer 2, 1x Lithium Buffer 3, 1x Lithium Buffer 4, 2x Lithium Buffer 5, 1x Lithium Regeneration Buffer 6

Lithium High Resolution Analytical Column
Item #:
80-6002-47

20cm x 4.6mm ID

Lithium High Performance Analytical Column
Item #:
80-6002-48

20cm x 4.6mm ID

Lithium Accelerated Analytical Column
Item #:
80-6002-49

20cm x 4.6mm ID

Lithium Prewash column
Item #:
80-6002-50

10cm x 4.6mm ID

Analytical Column Cleaning Service
Item #:
80-2112-17

All analytical columns

Prewash Column Cleaning Service
Item #:
80-2117-34

All prewash columns

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