Biochrom Bio 30+ In Vitro Diagnostic Amino Acid Analyzer Systems (EU, UK, Canada)

aaa 30+ amino acid analyzer biochrom

Biochrom Bio 30+ In Vitro Diagnostic Amino Acid Analyzer Systems (EU, UK, Canada)

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Biochrom’s Bio 30+ Series is the gold standard for amino acid analysis, trusted by laboratories in Europe, the United Kingdom, and Canada. This method, based on ion exchange chromatography with post-column derivatization with Ninhydrin, provides reliable qualitative and quantitative analysis of phenylalanine and tyrosine. The system is crucial for confirming and monitoring Phenylketonuria (PKU). The Biochrom Bio 30+ Physiological system utilizes 5 lithium citrate buffers, enabling a complete amino acid profile in under 2 hours through an accelerated method.

 

CE IVDR Declaration of Conformity Bio30+ Amino Acid Analyzer Series

DOWNLOAD BROCHURESCHEDULE A MEETINGdownload MSDS

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biochrom
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Why you need a dedicated instrument for amino acid analysis: 

Conventional HPLC systems face challenges in amino acid analysis due to sample complexity and unique chemical properties. A dedicated AAA system provides a reliable, consistent, and reproducible solution without the need for method development. Its pre-set and extensively tested configuration allows 24/7 routine use with minimal user intervention. Efficient screening of numerous samples for specific amino acids can be swiftly conducted around the clock using concise methods.

 

System suitable for: 

  • In Vitro Diagnostics for Phenylketonuria (PKU) purposes.

 

Specific applications:

  • Detection and monitoring of inborn errors of metabolism (IEM), such as PKU.
  • Detection of up to 56 amino acids in physiological samples for IVD purposes only

 

Biochrom 30+ Series of Amino Acid Analyzers are customizable.

Please contact your sales representative or distributor for system ordering information.

 product CE mark

 

Please contact your Biochrom representative for the service availability in your location.

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A Dedicated Amino Acid Analysis System 

Accurate: Superb separation, accurately identifies and quantifies free amino acids, as well as the amino acid composition of proteins and peptides.
Reliable: Minimal matrix interference and near baseline separation. Automated derivatization of amino acids maximizes reproducibility.
Fast: PKU screening under 35 mins.
Economical: The column cleaning and repacking service, gives columns an almost indefinite life and cuts the cost of ownership.
Fully Automated: Total PC control allows 24/7 operations.
Easy to implement: No lengthy method development is required as this well characterized system is established worldwide. All customers have their instrument optimized to their application directly from the factory.
Convenient: Interchangeable sample trays allow samples to be prepared in advance and stored refrigerated.
Robust: Advanced fluidics and robust hardware mean easy maintenance for users, backed by the reassurance of Biochrom's dedicated service and support team.
Flexible: Integrated software, choice of different methods to adapt to the user's needs. Automatic sample set-up. Networking and exporting of data.
Compact: Bench top design requiring minimum lab space.

 

Screening Methods

Choose from 3 full screening methods according to your business needs:

Choice of Screening Methods
System Use Injection to Injection
Biochrom Bio 30+ Physiological Accelerated LC System When speed is a decisive factor, with little compromise on the peak resolution 120 min
Biochrom Bio 30+ Physiological High Performance LC System The best compromise between speed, resolution and column life 170 min
Biochrom Bio 30+ Physiological High Resolution LC System When 100% baseline separation and a maximum number of amino acids separated are required. Ideal for low sample throughputs 210 min

 

 

Instrument Typical Package

The Instrument package consists of the Analyzer unit (electronics unit, pumps, and detection system) plus:

  • Cooled Autosampler
  • Column kit: analytical column and top-up ion exchange resin, prewash column and top-up ion exchange resin
  • Ninhydrin
  • Buffer bottles
  • Complete Chemical Kit suitable for up to 170 analyses (high performance method)
  • Spare parts and consumables kit
  • Calibration Standard
  • Hardware control and data handling software
  • Desktop PC, Windows 10 64-bit Professional, LED widescreen monitor
  • User Manual (PDF format)
  • Power cables

 

For buffer and chemicals information, consult the Consumables tab below.

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Instrument Specifications
Specification Data
Reproducibility Area: <1.5% RSD at 10 nanomoles.
Retention time: <0.5% RSD
Pumps Two dual piston pumps with integrated pressure transducer and drain valve
Ceramic pump head, sapphire pistons and valves
Electronically controlled piston wash
Operating Pressures Buffer pressure: maximum 150 bar
Ninhydrin pressure: maximum 24 bar
Analytical Column High pressure PEEK column packed with Ultropac 8 cation exchange resin
Peltier heating/cooling system
Sample Injection 3 injection modes (full loop, partial loop and micro), 84 position autosampler
Sample volumes from 1 µL to 5000 µL (200 µL loop supplied as standard)
Temperatures Column temperature variable between 20°C and 99°C
Reaction Coil temperature adjustable between 40°C and 145°C
(135°C nominal for UltraNynhydrin, 138°C nominal for EzNin)
Software BioSys Control Software
Biochrom Alias Manager Autosampler Control Software
OpenLAB CDS EZChrom Edition
Photometer Single flow cell with optical beam splitter with detection at 440 nm and 570 nm
Weight and Dimensions Main System: 50 kg (110 lb.) 48 x 59 x 57 cm (19 x 23 x 22 in.)
Autosampler: 21kg (46 lb.) 30 x 57.5 x 36 cm (12 x 23 x 14 in.)
Required Services Oxygen free nitrogen gas (99.99%) or argon gas regulated to 5 bar
Drainage facility
240V/100V, 50Hz/60Hz, 300VA mains supply
Safety Systems

Automatic shut down and reaction coil flushing in the event of:

  • Power failure
  • Photometer lamp failure
  • Low ninhydrin, low buffer, low coil or low nitrogen pressures
  • High coil or column temperatures, high ninhydrin, high buffer or high coil pressures

 

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Related Documents
Biochrom 30+ Amino Acid Analyzer System brochure.pdf DOWNLOAD
IVD Instructions for Use DOWNLOAD
Biochrom Bio 30+ Amino Acid Analyser User Manual DOWNLOAD
Biochrom Alias Autosampler User Guide DOWNLOAD
Material Safety Data Sheet (MSDS) View MSDS
Certificates of Analysis View Certificates of Analysis

 

Product name
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EZ Nin Reagent Kit 2L
Item #:
80-6000-13

Sufficient for up to 90 runs (Protein Hydrolysate System) or 45 runs (Physiological Fluid High Performance System) Includes: EZ Nin Reagent ready-to-use 2L bottle, In-line filter, Quick Start Guide

EZ Nin Reagent Kit 4L (2 x 2L)
Item #:
80-6000-14

Sufficient for up to 180 runs (Protein Hydrolysate System) or 90 runs (Physiological Fluid High Performance System) Includes: EZ Nin Reagent ready-to-use 2x 2L bottle, 2x In-line filter, Quick Start Guide

EZ Nin Reagent Kit 8L (4 x 2L)
Item #:
80-6000-15

Sufficient for up to 360 runs (Protein Hydrolysate System) or 180 runs (Physiological Fluid High Performance System) Includes: EZ Nin Reagent ready-to-use 4x 2L bottle, 4x In-line filter, Quick Start Guide

Ultra Ninhydrin Kit 2L
Item #:
80-2118-30

Sufficient for up to 90 runs (Protein Hydrolysate System) or 45 runs (Physiological Fluid High Performance System) Includes Ultra Ninhydrin Solution 2L, Ultrosolve Plus Solution, In-line Filter

Ultra Ninhydrin Kit 8L (4 x 2L)
Item #:
80-2117-76

Sufficient for up to 360 runs (Protein Hydrolysate System) or 180 runs (Physiological Fluid High Performance System) Includes 4x Ultra Ninhydrin Solution 2L, 4x Ultrosolve Plus Solution, 4x In-line Filter

Lithium Loading Buffer, Ph 2.20
Item #:
80-2038-10

2 L

Lithium Buffer 1, pH 2.80
Item #:
80-2038-15

2 L

Lithium Buffer 2, pH 3.00
Item #:
80-2038-16

2 L

Lithium Buffer 3, pH 3.15
Item #:
80-2099-83

2 L

Lithium Buffer 4, pH 3.50
Item #:
80-2097-18

2 L

Lithium Buffer 5, pH 3.55
Item #:
80-2037-69

2 L

Lithium Buffer 6
Item #:
80-2038-20

1 L

Physiological Fluid amino acid standard, 2.5mM
Item #:
80-6002-80

This ISO 17034 Reference Material (RM) is manufactured and verified in accordance with systemISO17034 and ISO17025 requirements. The analyte concentrations are verified by a quality control laboratory and the true value and uncertainty values at the 95% confidence level are reported for each analyte.

This is supplied in a screw cap glass vial containing 5 mL of standard solution in a 0.1 N Hydrochloric Acid matrix. A Certificate of Analysis is provided with each vial. Store Refrigerated (2o to 8oC) Do Not Freeze

Ultra Physiological Fluid Chemical Kit
Item #:
80-2117-77

Sufficient for up to 170 runs (High Performance System) Includes 2x Buffer 1, 1x Lithium Buffer 2, 1x Lithium Buffer 3, 1x Lithium Buffer 4, 2x Lithium Buffer 5, 1x Lithium Regeneration Buffer 6,1x Lithium Loading Buffer, Ultra Ninhydrin Kit 8 liters

Ultra Physiological Routine Kit
Item #:
80-6000-06

Sufficient for up to 170 runs (High Performance System) Includes 3x Buffer 1, 1x Lithium Buffer 2, 1x Lithium Buffer 3, 1x Lithium Buffer 4, 2x Lithium Buffer 5, 1x Lithium Regeneration Buffer 6, Ultra Ninhydrin Kit 8 liters

Lithium Buffer Start-up Kit (no ninhydrin reagent included)
Item #:
80-6000-16

Sufficient for up to 170 runs (High Performance System) Includes 2x Buffer 1, 1x Lithium Buffer 2, 1x Lithium Buffer 3, 1x Lithium Buffer 4, 2x Lithium Buffer 5, 1x Lithium Regeneration Buffer 6

Lithium Buffer Routine Kit (no ninhydrin reagent included)
Item #:
80-6000-17

Sufficient for up to 170 runs (High Performance System) Includes 3x Buffer 1, 1x Lithium Buffer 2, 1x Lithium Buffer 3, 1x Lithium Buffer 4, 2x Lithium Buffer 5, 1x Lithium Regeneration Buffer 6

Lithium High Resolution Analytical Column
Item #:
80-6002-47

20cm x 4.6mm ID

Lithium High Performance Analytical Column
Item #:
80-6002-48

20cm x 4.6mm ID

Lithium Accelerated Analytical Column
Item #:
80-6002-49

20cm x 4.6mm ID

Lithium Prewash column
Item #:
80-6002-50

10cm x 4.6mm ID

Analytical Column Cleaning Service
Item #:
80-2112-17

All analytical columns

Prewash Column Cleaning Service
Item #:
80-2117-34

All prewash columns

EMEA/UK Service Plans for Bio30+ Series of Amino Acid Analyzers
Preventative maintenance for Bio30+ systems
Item #:
80-2116-06
Silver Service Plan for Bio30+ systems
Item #:
80-6001-18
Gold Service Plan for Bio30+ systems
Item #:
80-2116-07
Platinum Service Plan for Bio30+ systems
Item #:
80-6000-08
IQ/OQ Documentation for AAA Bio30+
IQ/OQ for AAA with Biosys software (EMEA/UK only)
Item #:
90-6000-31
IQ/OQ for AAA with Phoenix software
Item #:
90-6003-40