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BTX User Spotlight: Unraveling Japanese encephalitis virus attenuation w/the BTX ECM 830

Unraveling Japanese encephalitis virus attenuation: Insights into vaccine development and viral pathogenicity

Song et al. (2025) conducted a comprehensive genetic analysis of the live-attenuated SA14-14-2 vaccine and its virulent parent strain SA14, identifying key viral factors responsible for their differences in pathogenicity, which could inform future antiviral strategies and broader neurotropic virus research. 

By Nick Price, PhD - May 23, 2025 ,
Infectious Disease & Vaccines

Japanese encephalitis virus remains a major cause of severe encephalitis in the Asia-Pacific region, and was recently detected in Europe and Africa raising global health concerns. Song et al. (2025) conducted a comprehensive genetic analysis of the live-attenuated SA14-14-2 vaccine and its virulent parent strain SA14, identifying key viral factors responsible for their differences in pathogenicity, which could inform future antiviral strategies and broader neurotropic virus research. 

In the study titled, "Key virulence factors responsible for differences in pathogenicity between clinically proven live-attenuated Japanese encephalitis vaccine SA14-14-2 and its pre-attenuated highly virulent parent SA14", the BTX ECM 830 electroporator was utilized to introduce viral RNA into cells. This electroporation technique facilitated the efficient delivery of genetic material, enabling the researchers to investigate the genetic determinants of virulence between the vaccine strain and its virulent parent. 

Full details are available here (PDF).

Learn more about the BTX ECM 830 Square Wave Electroporation System.
 


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